Genomics and Cell Biology Core

The goal of the Genomics and Cell Biology Core is to facilitate the application of cutting-edge genomics, bioinformatics, cell biology, and immunology techniques to nutrition and metabolic research.

Our ServicesOur Team

Getting started:

Interested in utilizing the services and equipment of the Genomics and Cell Biology Core at the NORCH? Hear from Dr. Ruslan Sadreyev on how to get started.

Core Services

Individual consultation and training services:

  • Strategic design of next-generation sequencing, nanoString, and microarray-based transcriptional profiling and other genomics related experiments

  • Illumina next-generation sequencing, including single-cell applications

  • Bioinformatic analysis of next-generation sequencing, nanoString, microarray, proteomics, and other large-scale data sets.

  • Variety of histologic, microscopic, cell biologic, tissue culture, and immunologic techniques and equipment

  • Specialized techniques in morphological, immunological, and tissue culture experiments

  • Cell culture-based techniques

  • Establishment of animal models for nutritional, food allergy, and obesity studies

Additional Services

  • Construction of next-generation sequencing libraries from submitted DNA or RNA

  • Construction of single-cell next-generation sequencing libraries for scRNA-seq, CITE-seq, scATAC-seq from submitted cell samples

  • Next-generation sequencing, with a major focus on RNA-Seq, metagenomics, ChIP-Seq, various single-cell platforms, and other experimental applications

  • Flow cytometry, cell sorting, phenotypic and functional analysis of lymphocytes, macrophages, dendritic cells, NK cells, and mast cells

  • Real-time-PCR and FACS for various transcription factors, cytokines, and chemokines and other immunologic assays

Workshops

  • Workshops on next-generation sequencing applications, bioinformatic analysis of high-throughput data, and other genomics-related procedures

  • Workshops on basic concepts in molecular biology and nutrition

Equipment

Click for the full list of equipment

  • Perkin Elmer fluorescence spectrometer
  • Inverted and epi fluorescence Zeiss microscopes
  • Nikon Eclipse fluorescence microscope
  • Union Biometrica COPAS BioSort robot
  • Titertek MapC2 Liquid and Agar Dispenser
  • Illumina HiSeq 2500
  • Illumina NextSeq 2000
  • Illumina MiSeq
  • Illumina CBot
  • 10 x Genomics Chromium
  • Covaris S2 shearing device
  • Agilent BioAnalyzer
  • BioRad qPCR
  • Flow cytometers (Attune NxT Flow Cytometer)
  • Vario-MACS immunomagnetic cell sorting unit
  • Beckman-Coulter DU-640 spectrophotometer
  • Neon Transfection System
  • ChemiDocMP Imaging System
  • SpectraMax ABS Microplate Readers
  • Luminex MAGPIX System

Inquiries and Scheduling

Upcoming Office Hours: Tues, Jan 26 10:30-4:30pm

 Dr. Sadreyev and Dr. Shi hold office hours on a quarterly basis for questions related to the services offered above. Zoom details can be requested via this inquiry form. 

Interested in utilizing our services or equipment for a current or upcoming project? Fill out the inquiry form below.

Core Leadership

Alessio Fasano, MD

Alessio Fasano, MD

Director of Genomics and Cell Biology Core
Allan Walker Chair in Pediatric Gastroenterology and
Nutrition
Ruslan Sadreyev, PhD

Ruslan Sadreyev, PhD

Primary Contact - Genomics Services

Associate Director of Genomics and Cell Biology Core
Instructor in Pathology

Phone: 617-643-5697
sadreyev@molbio.mgh.harvard.edu

Haining Shi, PhD, DVM

Haining Shi, PhD, DVM

Primary Contact - Cell Biology Services

Associate Director of Genomics and Cell Biology Core
Associate Professor of Pediatrics
Phone: 617-643-5697

CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE THE NORCH GRANT CONTRIBUTION IN THEIR PUBLICATIONS (P30DK040561)