Genomics and Cell Biology Core

The goal of the Genomics and Cell Biology Core is to facilitate the application of cutting-edge genomics, bioinformatics, cell biology, and immunology techniques to nutrition and metabolic research.
Primary Contact: Ruslan Sadreyev & Haining Shi (see info below) 

Core Leadership

Frederick Ausubel, PhD

Frederick Ausubel, PhD

Director of Genomics and Cell Biology Core
Professor of Genetics
Massachusetts General Hospital, Simches Research Center CPZN#7808
185 Cambridge St.
Boston, MA  02114
Phone: 617-726-5969; Fax: 617-726-5949
Alessio Fasano, MD

Alessio Fasano, MD

Co-Director of Genomics and Cell Biology Core
Allan Walker Chair in Pediatric Gastroenterology and Nutrition
Massachusetts General Hospital, Charlestown Navy Yard, 114 16th St.
Charlestown, MA  02129
Phone: 617-724-4604

Contact

Ruslan Sadreyev, PhD — PRIMARY CONTACT

Ruslan Sadreyev, PhD — PRIMARY CONTACT

Co-Associate Director of Genomics and Cell Biology Core
Instructor in Pathology
Massachusetts General Hospital, CPZN7
185 Cambridge St.
Boston, MA  02114
Phone: 617-643-5697
Haining Shi, PhD, DVM — PRIMARY CONTACT

Haining Shi, PhD, DVM — PRIMARY CONTACT

Co-Associate Director of Genomics and Cell Biology Core
Associate Professor of Pediatrics
Massachusetts General Hospital, Mucosal Immunology Laboratory, Room 3504
55 Fruit St.
Boston, MA  02114
Phone: 617-643-5697

Services

Consulting: Individual consultation and training for

Strategic design of next-generation sequencing, nanoString, and microarray-based transcriptional profiling and other genomics related experiments

Bioinformatic analysis of next-generation sequencing, nonoString, microarray, proteomics, and other genomics-related data sets.

Variety of histologic, microscopic, cell biologic, tissue culture, and immunologic techniques and equipment

Specialized techniques in morphological, immunological, and tissue culture experiments

Cell culture-based techniques

Establishment of animal models for nutritional, food allergy, and obesity studies

Construction of next-generation sequencing libraries from submitted DNA or RNA
Next-generation sequencing, with a major focus on RNA-Seq, metagenomics, ChIP-Seq, and other experimental applications
Choice of representative genes for nanoString transcriptional profiling analysis and nanoString analysis using the nanoString Preparation Station and “nCounter” Digital Analyzer
Shared antibody bank
Flow cytometry, cell sorting, phenotypic and functional analysis of lymphocytes, macrophages, dendritic cells, NK cells, and mast cells
Real-time-PCR and FACS for various transcription factors, cytokines, and chemokines and other immunologic assays
Workshops on next generation sequencing applications, bioinformatic analysis of high-throughput data, and other genomics-related procedures
Workshops on basic concepts in molecular cell biology and nutrition

Equipment

Perkin Elmer fluorescence spectrometer
Inverted and epi fluorescence Zeiss microscopes
STORM microscope
Nikon Eclipse fluorescence microscope
Union Biometrica COPAS BioSort robot
Titertek MapC2 Liquid and Agar Dispenser
Molecular Devices IXMicro automated microscope with robotic arm
Illumina HiSeq 2500
Illumina HiSeq 2000
Illumina MiSeq
Illumina CBot
Covaris S2 shearing device
Agilent BioAnalyzer
BioRad qPCR
nanoString Preparation Station and nCounter Digital Analyzer
Flow cytometers (FACScan and Accuri C6)
Vario-MACS immunomagnetic cell sorting unit
Beckman-Coulter DU-640 spectrophotometer
Neon Transfection System
Li-Cor imaging system

CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE THE NORCH GRANT CONTRIBUTION IN THEIR PUBLICATIONS (P30DK040561)